Cell Assay Senior Scientist - Blue Sky Kinases

We are looking for an experienced cell biologist to participate in our exciting studies of the human protein kinome.

This Senior Scientist position is part of the Blue Sky Initiative “ Seeing the Invisible in Protein Kinases ” at St. Jude Children's Research Hospital in Memphis, TN .

As a member of the Cell-Based Assays team, the candidate will use their molecular biology, biochemistry, and cell culture skills to perform research that translates novel insights regarding kinase structure and regulation in vitro into functional effects in cells. Additionally, the candidate will assist in performing cell-based analysis in high-, medium-, and low-throughput platforms of candidate inhibitors of kinases.

The work is diverse providing an opportunity to study multiple kinases, perform appropriate background research, optimize cell-based assays of various types using many different cell lines or primary cells. Not only are the cell-based studies used to validate the structural findings, but they are also used to discover new kinase-mediated biology or new mechanisms of kinase regulation.

The Blue Sky Initiative “ Seeing the Invisible in Protein Kinases ” is an inter-disciplinary project in the Department of Structural Biology and is supported by US $50 million in institutional funding. This large-scale project leverages ultra-high field nuclear magnetic resonance (NMR), complemented with cryoEM, X-ray crystallography, data-science and computational approaches, to characterize the conformational landscape of the human kinome. Knowledge of the conformations of kinases is then used to discover new biology regarding kinase function and regulation in cells, understand the structural and functional consequences of disease-associated mutations, and identify novel therapeutic opportunities.

Responsibilities :

Research kinase-mediated signaling pathways, kinase targets or kinase-dependent phosphorylation events, and cellular outcomes of such pathways for assigned kinase projects

Identify appropriate cellular assays and cells to perform structure-guided analysis of kinase signaling output and cellular phenotypes related to structure-guided perturbations in kinase regulation or function

Develop strategies and plans for implementing cell-based assays, including but not limited to evaluation of protein phosphorylation, protein interaction, protein localization, RNA abundance (qRT-PCR), protein ubiquitination, and protein abundance

Develop strategies and plans for implementing cell-based phenotypic assays, including but not limited to viability, apoptosis, autophagy, migration, or differentiation

Perform optimization and troubleshooting of cell-based assays

Perform statistical analysis using graphing software or R scripts

Interpret, analyze, and communicate cell-based assay results within the Cell-Based Assays team and with collaborators on specific kinase projects

Experience and Expertise:

Experience in analysis of protein kinases and phosphorylation-dependent signaling events or pathways.

Expertise in culturing a variety of types of cells, such as cancer cell lines, primary cells, mouse embryo fibroblasts, inducible human pluripotent cells, immune cells, neuronal cells, and muscle cells.

Expertise in multiple methods for exogenous protein expression.

Proficiency in presentation of experimental design and results using PowerPoint or similar software.

Experience with mouse models, preferred but not essential.

Technical Capabilities:

Cloning, PCR, and qRT-PCR

Sterile technique

Cell transfection, transduction, or infection to exogenously express proteins of interest transiently or for cell line generation

Quantitative immunoassays, such as ELISA, AlphaLISA, and Mesoscale Discovery

Immunoprecipitation and Western blotting

Protein-protein interaction analysis, such as proximity ligation, FRET, or NanoBit assays

Flow cytometry and fluorescence-activated cell sorting (FACS)

Reporter gene assays

Phenotypic assays, such as viability, apoptosis, proliferation, migration, colony-forming, autophagy, endocytosis, secretion, or differentiation assays

Microscopy, such as immunofluorescence, live-cell imaging, subcellular localization, colocalization analysis

Cellular fractionation

Competencies:

Creative and results-driven with strong critical thinking and the ability to learn quickly

Hardworking, well-organized, flexible, and capable of multitasking efficiently in a fast-paced environment

Collaborative and efficient member of teams with diverse backgrounds and opinions

Ability to prioritize work and complete assignments promptly

Minimum Education and Experience:

Bachelor's degree in relevant scientific area is required.

Minimum Experience:

Five (5) years of relevant work experience is required with a PhD.

Eight (8) years of relevant experience is required with a Master's Degree.

Ten (10) years of relevant work experience is required with a Bachelor's Degree.

The World’s Brightest Minds Always Innovate

At St. Jude Children’s Research Hospital, we know what can be achieved when the brightest scientific minds face the fewest barriers. That’s why we provide

World-class facilities

State-of-the-art technologies

Extraordinary support

A collaborative, bench-to-bedside environment

You will see, firsthand, how your science translates into survival for the children that we serve. Quite simply, at St. Jude, we encourage you to dream big and stop at nothing when it comes to finding cures and saving children.

Better at Work. Better at Life.

When you work at St. Jude, you’ll join a highly collaborative work culture that inspires you every day to be your best.  With opportunities for learning and growth, you can shape a career path that is right for you while also enjoying all the benefits and stability of working for a world-class institution. This includes work-life balance with generous paid time-off and on-campus conveniences that make life a little easier. Join us and you’ll quickly see why St. Jude is consistently ranked by our employees on Glassdoor as a “Best Place to Work.”

Have More in Memphis

Get the best of big-city amenities mixed with all the charm of a small-town feel when you live in Memphis. From our world-renowned music scene to our eclectic mix of food and people, Memphis is a place our employees are proud to call home. Our region enjoys a cost of living more than 20% lower than the national average and our state places no personal income tax on wages. Plus, Memphians spend five hours less per year in their daily commute compared to the national average. Learn how you can “ Have More in Memphis .”

Compensation In recognition of certain U.S. state and municipal pay transparency laws, St. Jude is including a reasonable estimate of the compensation range for this role. This is an estimate offered in good faith and a specific salary offer takes into account factors that are considered in making compensation decisions including but not limited to skill sets, experience and training, licensure and certifications, and other business and organizational needs. It is not typical for an individual to be hired at or near the top of the salary range and compensation decisions are dependent on the facts and circumstances of each case. A reasonable estimate of the current salary range is $104,000 - $186,160 per year for the role of Cell Assay Senior Scientist - Blue Sky Kinases.

Explore our exceptional benefits !

We are committed to a human-centered hiring experience. Technology may support portions of our process, but recruiting decisions involve human review and engagement. Learn more about our approach to AI .

St. Jude is an Equal Opportunity Employer

No Search Firms

St. Jude Children's Research Hospital does not accept unsolicited assistance from search firms for employment opportunities. Please do not call or email. All resumes submitted by search firms to any employee or other representative at St. Jude via email, the internet or in any form and/or method without a valid written search agreement in place and approved by HR will result in no fee being paid in the event the candidate is hired by St. Jude.