Researcher/Scientist III, Translational Cardiovascular Research Center - TCRC (Phoenix)

Plan, design and conduct advanced, multi-disciplinary research in collaboration with PI. Perform in vivo physiological examination to animals including, but not limited to: echocardiography, treadmill, Doppler, blood pressure. Isolation of mouse vessels, including the aorta, coronary artery, and perform vasodilation studies with myography. Manage the mouse colonies. Perform animal experiments (survival surgical procedure, e.g., open chest surgeries, contrast echocardiography, echocardiography, Doppler, treadmill, blood pressure measurement with a catheter,PV -loop) and data analysis. Measure the myocardial blood flow in vivo. Remain updated on literature of the related research topic and develop the projects aligning with the PI's research focus. Plan, coordinate, and conduct scientific research requiring judgment in the independent evaluation, selection, and substantial adaption and modification of standard scientific techniques. Design, perform, and/or oversee experiments and data collection to ensure data integrity, quality control, and protocol compliance. Report the experimental results along with original experiment data, protocol, analysis files (prism and ppt). Perform aspects related to cellular and molecular biology research that include, but are not limited to: bulk RNA-sequencing, scRNA-sequencing, bioinformatics, qRT-PCR, Immunoblotting, proteomics and metabolomics, and Immunocytofluorescence, primary cell isolation and culture, seahorse analysis, and flowcytometry. Attend and present research finding at regular group meetings and scientific conferences. Manage daily laboratory operations, including manage, maintain, and order laboratory equipment and supplies; lab inventories and respond to administrative request and procedures. Guide the work of others (students and trainees) and/or provides guidance to a specialized laboratory or research area. Serve as the bio-safety and chemical safety officer for TCRC, including the management of all lab safety and approval protocols. Develop and maintain detailed records of experiments, back the original data. Analyze data with statistical tools and writing findings narrative reports as appropriate. Prepare manuscripts for publication. Participate in the acquisition of research funding by initiating and writing grant proposals as appropriate. Assist with preparation of grants from the lab. Knowledge, Skills, and Abilities: Knowledge of In vivo physiological animal studies. Knowledge of microvessel isolation and myograph. Knowledge of molecular and cellular biology. Skilled in grant writing and manuscript preparation.