Research Assistant I- iPSC & Neuromuscular Disease Research

Position Summary/ Department Summary: We offer an opportunity to support cutting-edge neuromuscular disease research in the laboratory of Dr. Elizabeth Engle at Boston Children’s Hospital and Harvard Medical School ( http://kirbyneuro.org/EngleLab/ ). Our work uses human induced pluripotent stem cells (iPSCs), spinal cord organoids, skeletal muscle, and functional neuromuscular junction (NMJ) models to study spinal muscular atrophy (SMA) and therapeutic strategies that restore motor neuron–muscle connectivity. This position is ideal for a self-motivated and detail-oriented individual interested in developing a broad skill set in stem cell culture, organoid differentiation, microscopy, functional imaging, molecular biology, single-cell profiling, and drug screening. The successful candidate will join the Eagles Lab as a Laboratory Research Assistant I, working under the supervision of Dr. Kuchuan Chen. Evenings and weekend hours will occasionally be required for cell culture experiments. This position is looking for someone to start ASAP. Key Responsibilities: Human iPSC culture, expansion, cryopreservation, and routine quality control

Differentiation of iPSCs into spinal cord organoids and skeletal myogenic progenitors

Establishment and maintenance of human neuromuscular junction (NMJ) cultures and assembloids

Preparation and execution of functional NMJ drug screens and hit-validation experiments

Microscopy and live-cell functional imaging (i.e. fluorescence, confocal, GCaMP calcium imaging)

Cryosectioning, immunofluorescence staining, and quantitative image analysis

RNA purification, cDNA preparation, and quantitative PCR (qPCR)

Cell/nuclei dissociation, fluorescence activated cell sorting (FACS), and sample preparation for single-cell/single-nucleus profiling

Basic mouse work, including genotyping, tissue collection, and tissue processing

Preparation of media and reagents, maintenance of research equipment, and other routine lab tasks

Maintaining detailed records of experiments, cell lines, and analyses in lab notebooks and databases

Participation in weekly group meetings (including presenting research progress at lab meetings)

Minimum Qualifications Education: A bachelor's degree in STEM or Psychology is required for the role.

Preference for a degree in Biology, Neuroscience, Molecular Biology, Bioengineering, or a related field.

Experience: Experience with human iPSC/stem cell culture, neuron or muscle differentiation, or organoid culture

Familiarity with motor neuron, skeletal muscle, or other mammalian cell culture systems

Familiarity with immunostaining, cryosectioning, fluorescence/confocal microscopy, or live-cell imaging

Experience with qPCR/RNA purification, FACS/flow cytometry, high-throughput assays, or mouse models are pluses, but not required

Hands-on experience in sterile cell culture techniques, basic wet bench skills, and operating standard lab equipment

Ability to maintain and manage complex cell-culture workflows, including careful, reproducible aseptic technique and pipetting

The posted pay range is Boston Children’s reasonable and good-faith expectation for this pay at the time of posting.

Any base pay offer provided depends on skills, experience, education, certifications, and a variety of other job-related factors. Base pay is one part of a comprehensive benefits package that includes flexible schedules, affordable health, vision and dental insurance, child care and student loan subsidies, generous levels of time off, 403(b) Retirement Savings plan, Pension, Tuition and certain License and Certification Reimbursement, employee discounts and discounted commuter benefits at certain locations. Experience the benefits of passion and teamwork.