Research Scientist II

The Joag Lab studies tissue-resident memory CD8 T cells (Trm) which stand guard in our airways, lungs, and other mucosal tissues. We want to know how these sentinels change from childhood to adulthood, and how those changes shape our protection against respiratory infections. We are also turning T cells into tools: in fibrotic diseases, where scarred tissue shuts out antibodies and other therapies, we are exploring how T cells can open the door and help treatments reach their targets. The Joag lab was established in 2025, and this position offers the chance to shape lab trajectory and culture from the ground up.

Position summary

We are seeking a Research Scientist Level II to provide scientific leadership and technical execution of in vivo projects in lung fibrosis and T cell immunity against influenza. The position will involve in vivo mouse experiments, including viral infection models, bleomycin administration, in vivo lung-function testing (Flexivent), tissue extraction and enzymatic processing, flow cytometric analysis, and bioinformatics analysis. This person will have substantial experience in Immunology, Cell and Molecular Biology or related field and will help train and support other lab members.

Responsibilities

Plan and perform mouse infection experiments with respiratory and other viral pathogens, bleomycin administration (o.p. route) under ABSL-2 conditions

Administer substances via multiple routes, including oropharyngeal, intranasal, intravenous (tail vein), and intraperitoneal

Perform mouse dissections, extraction of various mucosal tissues, BAL, lymph nodes, lung perfusion, and lung function testing using Flexivent instrument

Carry out enzymatic and mechanical tissue digestion to generate single-cell suspensions

Plan and execute single-cell RNA sequencing experiments

Design, run, and analyze multiparameter flow cytometry experiments, including panel design, compensation, data analysis (e.g., FlowJo, Prism); FACS cell sorting

Ensure compliance with IACUC protocols, biosafety requirements, and institutional policies

Keep detailed, organized online laboratory records and maintain reagent and sample inventories

Present data at lab meetings and contribute to manuscripts and grant preparation

Train and support other lab members

Required Education and Experience Bachelor’s Degree in Immunology, Cell and Molecular Biology or related scientific discipline; may require a Master’s Degree for certain lab specialties. Minimum of two (2) years’ experience in a related area/laboratory setting.

Required Credentials N/A.

Preferred

Masters or PhD in Immunology, Cell and Molecular Biology or related scientific discipline.

Proficiency in multiparameter flow cytometry, cell sorting, and data analysis

mouse handling, and substance administration via various routes. Must be able to perform these tasks independently

ability to focus during long in vivo mouse experiments, tissue digests, flow cytometry and complex experimental planning

strong commitment to excellence, resilience, and good communication

Design experiments in collaboration with PI

Background in immunology, cell and molecular biology, or related field

Experience with ABSL-2 and BSL-2 work

Additional experience in one or more of the following areas is desirable:

Transcriptomic and/or genomic data analysis using R, Python, or related tools

Advanced immunofluorescence microscopy

Computational image analysis using ImageJ/Fiji, Python, Imaris or related tools

Personal qualities

Strong work ethic and commitment to rigorous, reproducible science

Exceptional attention to detail during all aspects of experimental work: design, execution, analysis, and record keeping

Highly organized, able to manage multiple time-sensitive experiments at once

Excellent and regular written and verbal communication with PI and other lab members

Professional, respectful, and collaborative with colleagues at all levels

maintain shared work calendar with lab members

Compensation Range $32.87 - $49.31 per hour

Salary Information

This compensation range was calculated based on full-time employment (2080 hours worked per calendar year). Offers are determined by multiple factors including equity, skills, experience, and expertise, and may vary within the range provided.

Disclaimer for Out of State Applicants

This compensation range is specific to Seattle, positions located outside of Seattle may be compensated differently depending on various factors .

Benefits Information

Seattle Children’s offers a generous benefit package, including medical, dental, and vision plans, 403(b), life insurance, paid time off, tuition reimbursement, and more. Additional details on our benefits can be found on our website www.seattlechildrens.org/careers/benefits .

About Us

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